Fragment Analysis
Fragment analysis can be performed up to 1200 base pairs specific to the regions on the genome to be analyzed. Fragments are stained with 6-FAM, VIC, NED, PED, LIZ fluorescent dyes and run on the Genetic Analyzer.
Results are analyzed with GeneMapper® Software v4 and v5. Base-pair sizes of the samples are measured according to the sizes of size standard that are used.
The study titles in which fragment analysis is used are as follows;
| MICROSATELLITE (STR) ANALYSIS | SNP GENOTYPING |
| linkage mapping – Animal breeding – Human, animal, and plant typing – Pathogen sub-typing – Genetic diversity – Microsatellite instability – Loss of Heterozygosity (LOH) – Inter-simple sequence repeat (ISSR) – Multilocus Variant Analysis (MLVA) | Linkage mapping – Animal breeding – Human, animal, and plant typing – Pathogen sub-typing – Genetic diversity – Microsatellite instability – Loss of Heterozygosity (LOH) – Inter-simple sequence repeat (ISSR) – Multilocus Variant Analysis (MLVA) |
| FINGERPRINTING | RELATIVE FLUORESCENCE |
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It is a method of isolating and displaying DNA sequences. Microbial genome typing – Animal or plant genome typing – Creation of genetic maps of new species – Genetic diversity and molecular phylogeny studies – Establishment of linkage groups among crosses
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Compare the peak sizes or areas covered by the peaks between two samples – Qualitative Fluorescence (QF) PCR – Quantitative Multiplex PCR of Short Fluorescent Fragments (QMPSF) – Multiplex Ligation-dependent Probe Amplification (MLPA)
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