Sanger Dizileme

Sanger Sequence Method

“Gold Standard” in DNA Sequence Analysis

In our DNA Sequence Analysis studies, we serve with our AB 3130 XL 16 Capillary and AB 3500 24 Capillary devices.

• We use Sequencing Analysis Software Sequencing – SeqScape® Software v2.7 in our Sequence Analysis.

What is the Sanger Sequence Method?

The genome is the entire hereditary code in the DNA of a living being, and the human genome consists of 6 billion base pairs. These base pairs are adenine (A), cytosine (C), guanine (G), and thymine (T). DNA sequencing method (Sanger Sequencing Method) is the gold standard among the methods used to determine the base sequence of a DNA fragment.

The DNA sequencing method, using by automated DNA sequencing devices, can be routinely applied in various genetic analysis studies by using chain termination technique with fluorescently labeled dideoxy nucleotides in combination with capillary electrophoresis technology.

In DNA sequencing, the DNA fragments to be sequenced are amplified in the PCR reaction with the help of DNA fragments called primers, which are the initiator of the sequence specific to the region to be amplified. In the sequencing device, the capillary extending between the + and – poles allows the negatively charged DNA to move from the – pole to the + pole. In a detector located near the + (anode) end of the capillary, the light reflected by fluorescent dyes excited by light from a laser lamp is recorded. The recorded data are mathematically evaluated and displayed with the software of the sequencing device. The electropherogram obtained after the sequencing, can be analyzed in analysis programs.